| 1 | Jearl Miles-Clark | USA | |
49,82 |
| 2 | Natasha Kaiser-Brown | USA | |
50,17 |
| 3 | Sandie Richards | JAM | |
50,44 |
| 4 | Tatyana Alekseyeva | RUS | 50,52 | |
| 5 | Ximena Restrepo | COL | 50,91 | |
| 6 | Sandra Myers | ESP | 51,22 | |
| 7 | Juliet Campbell | JAM | 51,40 | |
| Norfalia Carabalí | COL | DQ |