| 1 | Tatyana Firova | RUS | 51,36 | Q | |
| 2 | Novlene Williams-Mills | JAM | 51,77 | Q | |
| 3 | Aliann Pompey | GUY | 52,29 | Q | |
| 4 | Christine Amertil | BAH | 52,36 | ||
| 5 | Deedee Trotter | USA | 52,55 | ||
| 6 | Maris Mägi | EST | 53,30 |
| 1 | Debbie Dunn | USA | 52,08 | Q | |
| 2 | Vania Stambolova | BUL | 52,30 | Q | |
| 3 | Amantle Montsho | BOT | 52,34 | Q | NR |
| 4 | Natalya Nazarova | RUS | 52,47 | ||
| 5 | Denisa Rosolová | CZE | 52,92 | ||
| Bobby-Gaye Wilkins * | JAM | DQ |
* ... wegen Dopings nachträglich disqualifiziert