| 1 | Natalya Nazarova | RUS | 50,90 | Q | |
| 2 | Christine Amertil | BAH | 51,11 | Q | NR |
| 3 | Sviatlana Usovich | BLR | 52,18 | Q | |
| 4 | Sandie Richards | JAM | 52,20 | ||
| 5 | Aliann Pompey | GUY | 52,74 | ||
| 6 | Jennifer Meadows | GBR | 53,36 |
| 1 | Grit Breuer | GER | 51,56 | Q |
| 2 | Catherine Murphy | GBR | 51,74 | Q |
| 3 | Monique Hennagan | USA | 51,89 | Q |
| 4 | Grażyna Prokopek | POL | 53,07 | |
| Yuliya Pechenkina | RUS | DQ | ||
| Tonique Williams | BAH | DQ |