| 1 | Ionela Tirlea | ROM | 51,44 | Q |
| 2 | Hana Benesova | CZE | 52,38 | q |
| 3 | Carmo Tavares | POR | 53,06 | |
| 4 | Gulnara Safiulina | RUS | 53,28 |
| 1 | Helena Fuchsova | CZE | 52,27 | Q |
| 2 | Ester Goosens | NED | 52,52 | q |
| 3 | Patrizia Spuri | ITA | 53,21 | |
| 4 | Karen Shinkins | IRL | 53,45 |
| 1 | Grit Breuer | GER | 51,83 | Q |
| 2 | Irina Rosichina | RUS | 52,25 | q |
| 3 | Marie-Louise Bevis | FRA | 52,81 | |
| 4 | Kristina Perica | CRO | 54,55 | |
| 5 | Zana Minina | LTU | 55,82 |